ES cell technology services

Our services support the generation of mice carrying a disrupted or mutated specific genetic locus. To date, more than 40 projects have been completed successfully, including both obligatory and conditional knock-outs.

Generation of transgenic mice through:
Embryonic Stem Cell Gene Targeting, Blastocyst Injection, Chimera Production, Germline transmission.

DNA microinjection service

Our services support the generation of transgenic mice carrying an inserted cassette in their genome. To date, more than 70 transgenic lines have been successfully generated.

Generation of transgenic mice through:
DNA microinjection in the pronucleus, generation of founders. The service is certified with ISO 9001:2000 since 2008.

Archiving services

Our Unit is member of the European Mouse Mutant Archive Network. With more than 250 lines deposited in our CryoBank we guarantee the proper maintenance and distribution of cryopreserved material.

Storage, archiving and transportation of mutant strains through:
Embryo rederivation, cryopreservation and recovery of mouse embryo and sperm.
Since May 2010 we are part of the European Mouse Mutant Archive (EMMA) network. All services provided are in accordance with EMMA’s standards and protocols.

We provide services for BSRC Al. Fleming investigators as well as for collaborating scientists in academic Institutes or biotech/pharmaceutical companies worldwide.

Interested parties are welcome to qo through our procedure pages and order forms. Inquires for competitive prices can be forwarded to transgenics@fleming.gr .